Russell Brand
Dr. Nefario (voice)

Ken Jeong
Floyd (voice)

Steve Coogan
Silas (voice)

Moisés Arias
Antonio (voice)

Nasim Pedrad
Jillian (voice)

Kristen Schaal
Shannon (voice)

Pierre Coffin
Kevin the Minion / Bob the Minion / Stuart the Minion / Additional Minions / Evil Minions (voice)

Chris Renaud
Additional Minions / Evil Minions / Italian Waiter (voice)

Nickolai Stoilov
Arctic Lab Guards (voice)

Vanessa Bayer
Flight Attendant (voice)